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VOLUME 3 , ISSUE 4 ( October-December, 2020 ) > List of Articles

Original Article

Dynamics of Transferrin 2 (Tsf2) Expression in Various Developmental Stages of Culex quinquefasciatus

Vignesh Mariappan, Azhagu Rani Manoharan, Agieshkumar Balakrishna Pillai

Keywords : Culex quinquefasciatus, Transferrin, Transferrin 2, Vector immunity

Citation Information : Mariappan V, Manoharan AR, Pillai A B. Dynamics of Transferrin 2 (Tsf2) Expression in Various Developmental Stages of Culex quinquefasciatus. 2020; 3 (4):148-153.

DOI: 10.5005/jp-journals-10082-02273

License: CC BY-NC 4.0

Published Online: 09-02-2021

Copyright Statement:  Copyright © 2020; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Aim: Transferrin is an acute-phase protein known to be involved in insect immune responses. In the present study, we have assessed the transcript levels of transferrin 2 (Tsf2) in various developmental stages of vector mosquito Culex quinquefasciatus. Materials and methods: Culex quinquefasciatus mosquitoes were reared in laboratory conditions. Total RNA was extracted from larvae, pupae, and adult mosquitoes using the trizol method. Complementary DNA (cDNA) was synthesized and utilized for gene expression analysis using quantitative real-time PCR (qRT-PCR) and the result was expressed in the percentage fold change. Results: In this study, we observed an elevation in the fold change expression of Tsf2 in the larval to pupal stages followed by a decline in the fold of the adult stage. We speculate that the upregulation may be because of the microbial challenge or the aquatic stages are programmed to synthesis more Tsf2 to encounter the upcoming microbes present in the aquatic environment. However, the same should be ascertained by screening for the presence of microorganisms if any. Conclusion: The study presents a dynamic change in the expression of Tsf2 during the developmental process of mosquito. The role of the protein in vector competence deserves further studies.


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